What was the rationale of using 32P and 35S by Hershey and Chase ? Instead, if we use radiolabelled C and N, will the results be any different ?
Phosphorus is present in the backbone of the DNA strands and Sulphur is constituent of amino acids or protein thus rationale of this experiment was to differentiate between DNA and protein.
If we use radiolabelled C and N then we cannot diffferentiate as both DNA and protein have C and N, so both will be labelled.
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