Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease.

Gel electrophoresis is the technique that is used to separate the DNA fragments formed by the use of restriction endonucleases.

In this technique the DNA fragments are separated on the basis of the charge , since the DNA molecule is negatively charged they are forced to move towards the anode under an electric field applied through the medium or the matrix in which the DNA is loaded. The matrix used is generally made of agarose.

i. The DNA fragments cut by the restriction enzymes, separate according to their size through the sieving effect provided by the agarose gel.

ii. The smaller the DNA fragment the faster it travels. Smaller segments travel more distance than larger DNA molecules. 

iii. The DNA which is usually stained with the EtBr dye or ethidium bromide,  is visualized under UV light. The DNA appear as orange bands.