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Molecular Basis of Inheritance

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Biology

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Biology

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CBSE Gujarat Board Haryana Board

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Class 10 Class 12

Silencing of mRNA has been used in producing transgenic plants resistant to

  • bollworms

  • nematodes

  • White rusts

  • bacterial blight


B.

nematodes

RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. This method involves silencing of a specific mRNA due to a complementary ds RNA molecule that binds to an prevents translation of the mRNA (silencing). The source of the complementary RNA could be from an infection by viruses having RNA genome of mobile genetic elements (transposons) that replicate via an RNA intermediate. Using Agrobacterium vectors, nematode -specific genes were introduced into the host plant. The introduction of DNA was such t hat it produced both sense and anti -sense RNA in the host cells. These two RNA's being complementary to each other formed a dsRNA that initiated RNAi and thus, silenced the specific mRNA of the nematode. The result was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite. 

RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. This method involves silencing of a specific mRNA due to a complementary ds RNA molecule that binds to an prevents translation of the mRNA (silencing). The source of the complementary RNA could be from an infection by viruses having RNA genome of mobile genetic elements (transposons) that replicate via an RNA intermediate. Using Agrobacterium vectors, nematode -specific genes were introduced into the host plant. The introduction of DNA was such t hat it produced both sense and anti -sense RNA in the host cells. These two RNA's being complementary to each other formed a dsRNA that initiated RNAi and thus, silenced the specific mRNA of the nematode. The result was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite. 

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List the function of RNA.

Functions of RNA
RNA acts as genetic material in viruses
It also functions as an adapter and messenger 
It also acts as a catalytic molecule and catalyses various biochemical reactions.
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How many nucleotides are present in a bacteriophage ϕ x 174.

5386
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Comment two chains of DNA have antiparallel polarity.

The Two chain of DNA are antiparallel that is
if one chain has 5’ x 3’ polarity the other chain has 3’ x 5’ polarity.
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List the number of base pairs in :

(i) lambda bacteriophage

(ii) E.coli and

(iii) haploid content of human DNA.


(i) 48502 bp
(ii) 4.6 x 106 bp and
(iii) 3.3 x 109 bp.
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Name the genetic material for majority of organisms.

DNA (Deoxyribose nucleic acid)
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