Write a note on vectors used during recombinant DNA technology. D

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 Multiple Choice QuestionsLong Answer Type

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51.

Write a note on vectors used during recombinant DNA technology. Draw diagram of any two vectors


A vector or vehicle DNA is used as carrier for transferring selected DNA into cells. A plasmid with its small DNA from a bacterium is a good choice for indirect gene transfer, because it can move from one cell to another and make several copies of itself. However, artificial chromosomes from bacteria and yeast called BACs and YACs respectively are more efficient for eukaryotic gene transfers.

Plasmid and Yeast Artificial Chromosome

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 Multiple Choice QuestionsShort Answer Type

52. How and why is the bacterium Thermus aquaticus employed in recombinant DNA techonolgy ? Explain.
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53. Name the source of Taq polymerase. Explain its advatages.
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54. What are recombinant proteins ? How do bioreactors help in their production ?
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55. How is  Fragmentation of DNA of interest carried out in recombinant DNA technology ?
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 Multiple Choice QuestionsMultiple Choice Questions

56. Genetic engineering would not have been possible if which of the following were not known ?
  • DNA polymerase
  • RNA synthetase
  • DNA ligase
  • Reverse transcriptase
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57. Genetic engineering is possible because:
  • Phenomenon of transduction in bacteria is well understood
  • We can see DNA by electron microscope
  • We can cut DNA at random sites.
  • Restriction endonucleases purified from bacteria can be used in vitro
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58. Restriction endonucleases are :
  • Synthesized by bacteriophages
  • Present in mammalian cells for degradation of DNA
  • Used for in vitro DNA synthesis
  • Used in genetic engineering
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59. Structure involved in genetic engineering is :
  • Plasmid 
  • Codon
  • Plastid 
  • Scissors
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60. Which enzyme is useful in genetic engineering ?
  • DNAase
  • Amylase
  • Lipase 
  • Restriction endonuclease
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