Name the source of Taq polymerase. Explain its advatages. from Bi

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 Multiple Choice QuestionsLong Answer Type

51.

Write a note on vectors used during recombinant DNA technology. Draw diagram of any two vectors

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 Multiple Choice QuestionsShort Answer Type

52. How and why is the bacterium Thermus aquaticus employed in recombinant DNA techonolgy ? Explain.
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53. Name the source of Taq polymerase. Explain its advatages.


Taq Polymerase is extracted from the  bacteria namely Thermus aquaticus. It remains active at higher temperature and is used for replicate short DNA segments.
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54. What are recombinant proteins ? How do bioreactors help in their production ?
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55. How is  Fragmentation of DNA of interest carried out in recombinant DNA technology ?
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 Multiple Choice QuestionsMultiple Choice Questions

56. Genetic engineering would not have been possible if which of the following were not known ?
  • DNA polymerase
  • RNA synthetase
  • DNA ligase
  • Reverse transcriptase
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57. Genetic engineering is possible because:
  • Phenomenon of transduction in bacteria is well understood
  • We can see DNA by electron microscope
  • We can cut DNA at random sites.
  • Restriction endonucleases purified from bacteria can be used in vitro
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58. Restriction endonucleases are :
  • Synthesized by bacteriophages
  • Present in mammalian cells for degradation of DNA
  • Used for in vitro DNA synthesis
  • Used in genetic engineering
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59. Structure involved in genetic engineering is :
  • Plasmid 
  • Codon
  • Plastid 
  • Scissors
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60. Which enzyme is useful in genetic engineering ?
  • DNAase
  • Amylase
  • Lipase 
  • Restriction endonuclease
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