What is the basic purpose of translation from Biology Molecular B

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76.

How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

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77. List the characteristics of DNA molecule.
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78.

Describe briefly the mechanism of DNA replication.

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79.

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80.

What is the basic purpose of translation


Protein Synthesis

It consists of two main events :

(A) Transcription and (B) Translation.

Transcription. It is the copying of a complementary messenger RNA strand on DNA strand, the strand which acts as template is termed master strand or sense strand. The base pairing follow A = U and G=C. In eukaryotes transcription occurs in the nucleus. The mRNA synthesized come out into cytoplasm through nuclear pore.

Transcription requires a template (double stranded DNA), ribonucleoside triphosphates (ATP, GTP, CTP and UTP (RNA polymerase II and divalent metal ion.)

RNA chain starts at promoter region and ends at terminator region and synthesized in 5’→3’ direction. Chain termination is brought by Rho factor.

A Fig. 2.11—A. Sigma factor and coenzyme join to form RNA polymerase.

B. RNA polymerase attaches to initiation site 8 factor help in detecting the promoter or initiation site.

C. DNA unwinds, core enzyme catalyzes the synthesis of mRNA 8 factor separates out.

D. mRNA chain elongates.

E. Termination of chain brought by 8 factors.

F. mRNA strand transcribed by DNA.

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