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Biotechnology : Principles and Processes

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Biology

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Biology

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CBSE Gujarat Board Haryana Board

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Class 10 Class 12

Restriction endonucleases are enzymes which

  • make cuts at specific positions within the DNA molecule

  • recognize a specific nucleotide sequence for binding of DNA ligase

  • restrict the action of the enzyme DNA polymerase

  • remove nucleotides from the ends of the DNA


A.

make cuts at specific positions within the DNA molecule

Restriction endonucleases recognise a specific DNA base sequence (recognition sequence of the recognition site, restriction sequence or restriction site) and cleave both the strands of DNA at or near the site. The enzyme cuts are DNA, generating restriction fragments wit overhanging ends or blunt ends.

Restriction endonucleases recognise a specific DNA base sequence (recognition sequence of the recognition site, restriction sequence or restriction site) and cleave both the strands of DNA at or near the site. The enzyme cuts are DNA, generating restriction fragments wit overhanging ends or blunt ends.

372 Views . 7 Shares

Define recombinant DNA.

Recombinant DNA is the artificially created DNA which incorporates DNA segments from different sources into one.
852 Views . 6 Shares

What are BACs and YACs ?

BACs and YACs are artificial chromosomes from bacteria and yeast used for efficient gene transfer and gene sequencing.
970 Views . 8 Shares

Name the soil bacterium which contain gene for production of endotoxins.

Bacillus thuringiensis.
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What is the role of restriction endonuclease ?

Restriction endonucleases are specific enzymes which can cleave double stranded DNA at specific sites called recognition sequences. Restriction endonucleases provide protection to the bacteria by restricting the growth of bacteriophages. They also serve as tools for Recombinant DNA technology.

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What is genetic engineering ?

Genetic engineering is a technique for artificially and deliberately modifying DNA (genes) to suit human needs. It is used to change the genetic makeup of the cells by using Recombinant DNA technology 
1228 Views . 3 Shares

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