191.

The sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme

• Not 1

• Eco R1

• Hae III

• Puv I

Paul berg's gene splicing experiment created the first rDNA molecule, which was a

• T4 phage fragment incorporated into SV40 vector

• Lambda phage fragment incorporated into SV40 vector

• T4 phage fragment incorporated into pSC 101 vector

• Lambda phage fragment incorporated into pSC 101 vector

Viral genome incorporated into host DNA is called

• prophase

• plasmid

• prophage

• bacteriophage

Recognition sequence in Eco RI is

• GGCC

• GAATTC

• AAGCTT

• CTGCAG

In lysogenic phase which of the following takes place?

• Phage DNA integrated with host genome and does not multiplied

• Phage DNA does not integrate with host genome

• Phage DNA integrates with host genome and bacterial cell does not lyse

• Phage DNA integrated with host DNA and multiplied

Science of engineering and technology applied to life sciences is

• Biotechnology

• Genetic engineering

• Pathology

• Biological science

Plasmid is

• small extrachromosomal circular self replicating DNA that can carry genes into host organism

• bacteriophage

• DNA found in mitochondria

• DNA incorporated in bacterial chromosome

e -DNA is formed from m-RNA by which enzyme:

• restriction endonuclease

• reverse transcriptase

• DNA polymerase

• RNA polymerase

Hybridoma technology is used to :

• kill the cancer cells

• formation of somaclonal antibodies

• formation of somatic hybrids

• formation of antibiotics

Kohler and Milstein developed biotechnology for :

• monoclonal antibodies

• steroid synthesis

• immobilization of enzymes

• myeloma