Kohler and Milstein developed biotechnology for : from Biology B

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 Multiple Choice QuestionsMultiple Choice Questions

191.

The sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme

  • Not 1

  • Eco R1

  • Hae III

  • Puv I


192.

Paul berg's gene splicing experiment created the first rDNA molecule, which was a

  • T4 phage fragment incorporated into SV40 vector

  • Lambda phage fragment incorporated into SV40 vector

  • T4 phage fragment incorporated into pSC 101 vector

  • Lambda phage fragment incorporated into pSC 101 vector


193.

Viral genome incorporated into host DNA is called

  • prophase

  • plasmid

  • prophage

  • bacteriophage


194.

Recognition sequence in Eco RI is

  • GGCC

  • GAATTC

  • AAGCTT

  • CTGCAG


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195.

In lysogenic phase which of the following takes place?

  • Phage DNA integrated with host genome and does not multiplied

  • Phage DNA does not integrate with host genome

  • Phage DNA integrates with host genome and bacterial cell does not lyse

  • Phage DNA integrated with host DNA and multiplied


196.

Science of engineering and technology applied to life sciences is

  • Biotechnology

  • Genetic engineering

  • Pathology

  • Biological science


197.

Plasmid is

  • small extrachromosomal circular self replicating DNA that can carry genes into host organism

  • bacteriophage

  • DNA found in mitochondria

  • DNA incorporated in bacterial chromosome


198.

e -DNA is formed from m-RNA by which enzyme:

  • restriction endonuclease

  • reverse transcriptase

  • DNA polymerase

  • RNA polymerase


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199.

Hybridoma technology is used to :

  • kill the cancer cells

  • formation of somaclonal antibodies

  • formation of somatic hybrids

  • formation of antibiotics


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200.

Kohler and Milstein developed biotechnology for :

  • monoclonal antibodies

  • steroid synthesis

  • immobilization of enzymes

  • myeloma


A.

monoclonal antibodies

Monoclonal antibodies are monospecific antibodies that are made by identical immune cells that are all clones of a unique parent cell.

In 1975, Georges Kohler and Cesar Milstein succeeded in making fusions of myeloma cell lines with B cells to produce hybridomas that made antibodies to known antigens. These are pure, high affinity antibodies, specific for an antigen which are obtained outside the body from clonal cultures of hybridoma cells.


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