Multiple Choice QuestionsThe sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme
Not 1
Eco R1
Hae III
Puv I
Paul berg's gene splicing experiment created the first rDNA molecule, which was a
T4 phage fragment incorporated into SV40 vector
Lambda phage fragment incorporated into SV40 vector
T4 phage fragment incorporated into pSC 101 vector
Lambda phage fragment incorporated into pSC 101 vector
Viral genome incorporated into host DNA is called
prophase
plasmid
prophage
bacteriophage
C.
prophage
After infecting the bacterium, the virus can follow lysogenic cycle in which bacterial DNA is not destroyed, instead the viral DNA is incorporated into it and attached to the bacterial DNA in a specific manner and replicates along with this. Bacteria which carry a provirus are called lysogenic bacteria. In this condition the viral DNA is transmitted to the progeny of bacteria such a virus is known as provirus are prophage.
In lysogenic phase which of the following takes place?
Phage DNA integrated with host genome and does not multiplied
Phage DNA does not integrate with host genome
Phage DNA integrates with host genome and bacterial cell does not lyse
Phage DNA integrated with host DNA and multiplied
Science of engineering and technology applied to life sciences is
Biotechnology
Genetic engineering
Pathology
Biological science
Plasmid is
small extrachromosomal circular self replicating DNA that can carry genes into host organism
bacteriophage
DNA found in mitochondria
DNA incorporated in bacterial chromosome
e -DNA is formed from m-RNA by which enzyme:
restriction endonuclease
reverse transcriptase
DNA polymerase
RNA polymerase
Hybridoma technology is used to :
kill the cancer cells
formation of somaclonal antibodies
formation of somatic hybrids
formation of antibiotics
Kohler and Milstein developed biotechnology for :
monoclonal antibodies
steroid synthesis
immobilization of enzymes
myeloma
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