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Biotechnology and Its Applications

Short Answer Type

31. List two uses of cloned genes in molecular diagnostics.

Uses of cloned gene in molecular diagnosis.

1. Cloned genes can be used as probes to detect the mutated gene responsible for the disease from a pool of molecules.

2. Cloned gene can be used to detect antibodies or antigens in infections. Like in ELISA which is based on antibody-antigen interaction.

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32. How is ELISA test carried out ?

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33. Name two of common pharmaceutically important products obtained through genetic engineering.

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Long Answer Type

34.

How insulin is synthesised in human (or mammals) . Give a suitable diagram?

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35.

How is E.coli used to produce human insulin?

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Short Answer Type

36. What is gene library ?

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37. Define probes.

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38. Why is the use of probes considered better than conventional diagnostic tools for disease diagnosis ?

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39. Why mice are considered most suitable animals for transgenic production ?

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40. Write the advantages of recombinant therapeutics.

How many of them have been approved world over for human use and how many are available in Indian market ?

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