How did an American Company. Eli Lilly use the knowledge of r-DN

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 Multiple Choice QuestionsShort Answer Type

91.

How can bacterial DNA be released from the bacterial cell for biotechnology experiments?

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92. Explain how a hereditary disease can be corrected. Give an example of first successful attempt made towards correction of such diseases.
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93. Why do children cured by enzyme-replacement therapy for adenosine deaminase deficiency need periodic treatment?
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94. Explain the significance of satellite DNA in DNA fingerprinting technique.
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95.

Describe any three potential applications of genetically modified plants.

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96. How did an American Company. Eli Lilly use the knowledge of r-DNA technology to produce human insulin?


American Company Eli Lilly used the knowledge of r-DNA technology to produce human insulin in the following way.

1. Preparation of DNA corresponding to A and B chain separately
2. Extraction of plasmid from bacteria
3. Insertion of DNA corresponding to A and B chain separately in plasmid
4. Transforming the bacteria with this recombinant plasmid
5. Expression of the desired product from this DNA .
6. Purification of A and B chain
7. Linking them with disulfide bonds so that they act as human insulin
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97.

State the role of C-peptide in human insulin.

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98.

How have transgenic animals proved to be beneficial in:
(a) Production of biological products
(b)Chemical safety testing.

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 Multiple Choice QuestionsLong Answer Type

99.

With advancements in genetics, molecular biology and tissue culture, new traits have been incorporated into crop plants. Explain the main steps in breeding a new genetic variety of a crop. 

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 Multiple Choice QuestionsShort Answer Type

100.

(a) Mention the cause and the body system affected by ADA deficiency in humans.

(b) Name the vector used for transferring ADA-DNA into the recipient cells in humans. Name the recipient cells. 
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