Miller's experiment. Miller (1953) made the first successful simulation experiment to assess the validity of the claim for the origin of organic molecules. Miller sealed in a spark chamber a mixture of water, methane, ammonia, hydrogen gas. He made arrangement for boiling water. The trap in turn, was connected with the flask for boiling water. After 18 days, significant amount of simple major organic compounds such as amino acids such as glycine, alanine, and aspartic acid and peptide chains, began to appear. Simple sugars, urea, short chain fatty acids were also formed. In atmosphere, this spark is provided by U.V. light or other energy source.

Stanley Miller's Experiment in the artificial production of organic compounds.