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Molecular Basis of Inheritance

Short Answer Type

41. Write differences between Prokaryotic DNA and Eukaryotic DNA.

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42.

Differentiate between the followings:
(a) Repetitive DNA and Satellite DNA
(b) mRNA and tRNA
(c) Template strand and Coding strand

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43. Which property of DNA double helix led Watson and Crick to hypothesize semi-conservative mode of DNA replication ? Explain.

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44. Differentiate Leading Strand and Lagging Strand.

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Long Answer Type

45.
Sketch and explain clover leaf model of tRNA.

Transfer RNA or soluble RNA (tRNA or sRNA).

It constitutes 15% of total RNA and is smallest out of three with only 70-85 nucleotides having sedimentation coefficient 45.

It is of 100 types. Nitrogen bases of some nucleotides are modified to provide coiling to the otherwise single stranded RNA. The tRNA has two models clover leaf and L-form model. It has different specific loops as Shown :

tRNA is meant for transferring specific amino acid to the ribosomes for synthesis of polypeptides. tRNAs are synthesized as usual on DNA template however, their 3’ end having CCA sequence is added after the transcription.

Clover leaf model of tRNA.

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