Multiple Choice QuestionsUse of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
Bio-infringement
Biopiracy
Bioexploitation
Biodegradation
B.
Biopiracy
Biopiracy is a term used for or refers to the use of bioresources by multinational companies and other organisation without proper authorisation from the countries and people concerned with compensatory payment.
The DNA fragments separated on an agarose gel can be visualised after staining with
Bromophenol blue
Acetocarmine
Aniline blue
Aniline blue
D.
Aniline blue
Ethidium bromide is used to stain the DNA fragments and will appear as orange coloured bands under UV light.
In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is
Indian Council of Medical Research (ICMR)
Council for Scientific and Industrial Research (CSIR)
Genetic Engineering Appraisal Committee (GEAC)
Research Committee on Genetic Manipulation (RCGM)
C.
Genetic Engineering Appraisal Committee (GEAC)
Indian Government has setup organisation such as GEAC (Genetic Engineering Appraisal Committee) which will make decisions regarding the validity of GM research and safety of introducing GM-organism for public services.
Which of these statements is incorrect?
Enzymes of TCA cycle are present in mitochondrial matrix
Glycolysis occurs in cytosol
Oxidative phosphorylation takes place in outer mitochondrial membrane
Glycolysis operates as long as it is supplied with NAD that can pick up hydrogen atoms
C.
Oxidative phosphorylation takes place in outer mitochondrial membrane
Oxidative phosphorylation takes place in inner mitochondrial membrane.
The sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme
Not 1
Eco R1
Hae III
Puv I
C.
Hae III
Hae III is extracted from Haemophiles influenzae it cuts G-C in 5'-3' direction in both templates.
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
If both assertion and reason are true and reason is the correct explanation of assertion.
If both assertion and reason are true but reason is not the correct explanation of assertion.
If assertion is true but reason is false.
If both assertion and reason are false.
C.
If assertion is true but reason is false.
Restriction enzyme, a type of endonuclease, functions by "inspecting" the length of a DNA sequence. Once it finds a recognition sequence, it binds and cuts each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counter parts, i.e. they can join similar complementary end of DNA fragment from some other source with the help of DNA ligase. This stickiness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.
Paul berg's gene splicing experiment created the first rDNA molecule, which was a
T4 phage fragment incorporated into SV40 vector
Lambda phage fragment incorporated into SV40 vector
T4 phage fragment incorporated into pSC 101 vector
Lambda phage fragment incorporated into pSC 101 vector
B.
Lambda phage fragment incorporated into SV40 vector
Paul Berg's gene splicing experiment created the first rDNA molecule, which was a lambda phage fragment incorporated into SV40 vector.
All monerons
Contain DNA and RNA
Demonstrate a long circular strand of DNA, not formed enclosed in a nuclear membrane
Are bacteria
All of the above
D.
All of the above
The correct order of steps in Polymerase Chain Reaction (PCR) is
Extension, Denaturation, Annealing
Annealing, Extension, Denaturation
Denaturation, Annealing, Extension
Denaturation, Extension, Annealing
C.
Denaturation, Annealing, Extension
PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.
A gene whose expression helps to identify transformed cell is known as
Selectable marker
Vector
Plasmid
Plasmid
A.
Selectable marker
