The usual source of restriction endonucleases used in gene cloning is from

• fungi

• bacteria

• plants

• viruses

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

• centrifugation

• polymerase chain reaction

• electrophoresis

• restriction mapping

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

• Non-recombinant bacteria containing $\mathrm{\beta }$-galactosidase

• lnsertional inactivation of $\mathrm{\alpha }$-galactosidase in non-recombinant bacteria

• lnsertional inactivation of $\mathrm{\alpha }$-galactosidase in recombinant bacteria

• Inactivation of glycosidase enzyme in recombinant bacteria

During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is

• DNA ligase

• restriction endonuclease

• alkaline phosphatase

• exonuclease

The structure of E. coli chromosomal DNA is

• double- stranded, right handed and circular

• single- stranded, right handed and circular

• double- stranded, left handed and linea

• double- stranded, left handed and circular

Passage cells help in

• transport of water towards pericycle

• transport of water towards epiblema

• absorption of water from soil

• passage of CO₂ towards stomata

The sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme

• Not 1

• Eco R1

• Hae III

• Puv I

Paul berg's gene splicing experiment created the first rDNA molecule, which was a

• T4 phage fragment incorporated into SV40 vector

• Lambda phage fragment incorporated into SV40 vector

• T4 phage fragment incorporated into pSC 101 vector

• Lambda phage fragment incorporated into pSC 101 vector

Viral genome incorporated into host DNA is called

• prophase

• plasmid

• prophage

• bacteriophage

100.

Recognition sequence in Eco RI is

• GGCC

• GAATTC

• AAGCTT

• CTGCAG