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Biotechnology : Principles and Processes

One Word Answers

11. What is the source of DNA polymerase i.e., Taq polymerase ?

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Short Answer Type

12. Define “melting of target DNA”.

The target DNA containing sequence to be amplified is heat denatured (around 94°C for 15 seconds) to separate its complementary strands. the high temperature causes the hydrogen bond to break and thus the strands are separated and this is called melting of target DNA.

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13. What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

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14. Do eukaryotic cells have restriction endonucleases ? Justify your answer.

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15. From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

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One Word Answers

16. Expand EFB.

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Short Answer Type

17. What are two core techniques that enabled birth of biotechnology ?

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Long Answer Type

18.

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

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Short Answer Type

19. List three important features necessary for preparing genetically modified organism.

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20. Make a list of tools of recombinant DNA technology.

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