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Long Answer TypeWhat is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.
Steps in genetic engineering:
1. Identification and isolation of agronomically important genes.
2. Cloning of isolate genes in a vector.
3. Introduction of gene into plaint protoplast cell / tissues with the use of gene transfer method.
4. Culture and regeneration complete plant on suitable selection medium.
5. Integration of foreign gene in the transgenic plants by using molecular techniques.
â–² Fig. 7.1. Diagram showing Various steps involved in DNA recombinant technology for the production of a recombinant protein.
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