14.
Do eukaryotic cells have restriction endonucleases ? Justify your answer.
No, eukaryotic cells do not have restriction endonucleases. This is because the DNA of eukaryotes is highly methylated by a modification enzyme, called methylase. Methylation protects the DNA from the activity of restriction enzymes .These enzymes are present in prokaryotic cells where they help prevent the invasion of DNA by virus.
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15.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?
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One Word Answers
16.
Expand EFB.
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Short Answer Type
17.
What are two core techniques that enabled birth of biotechnology ?
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Long Answer Type
18.
What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology. Or With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.
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Short Answer Type
19.
List three important features necessary for preparing genetically modified organism.
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20.
Make a list of tools of recombinant DNA technology.