What are two core techniques that enabled birth of biotechnology

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 Multiple Choice QuestionsOne Word Answers

11. What is the source of DNA polymerase i.e., Taq polymerase ?
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12. Define “melting of target DNA”.
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13. What would be the molar concentration of human DNA in a human cell ? Consult your teacher.
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14. Do eukaryotic cells have restriction endonucleases ? Justify your answer.
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15. From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?
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16. Expand EFB.
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17. What are two core techniques that enabled birth of biotechnology ?


The two core techniques that enabled birth of modern biotechnology are :

1. Genetic engineering—Techniques to alter the makeup of genetic material (DNA and RNA), by introducing new genes into host organisms and thus change the phenotype of the host organism.

2. Maintenance of sterile (microbial contamination-free) environment in chemical engineering processes to enable growth of only the desired cell in large quantities for the manufacture of biotechnological products like antibiotics, vaccines, enzymes etc.

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 Multiple Choice QuestionsLong Answer Type

18.

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

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 Multiple Choice QuestionsShort Answer Type

19. List three important features necessary for preparing genetically modified organism.
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20. Make a list of tools of recombinant DNA technology.
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