Short Answer TypeHow has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in the correct sequence.
A nematode Meloidogyne incognita infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi).
Using Agrobacterium vectors, nematode-specific genes were introduced into the host plants. The introduction of DNA was such that it produced both sense and antisense RNA in the host cells. These two RNA’s being complementary to each other formed a double-stranded (dsRNA) that initiated RNAi and thus, silenced specific mRNA of the nematode.
The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite.
Explain the roles of the following with the help of an example each in recombinant DNA technology:
(a) Restriction Enzymes
(b) Plasmids
Multiple Choice QuestionsWhich of the following is not a feature of the plasmids?
Circular structure
Transferable
Single stranded
Single stranded
The Taq polymerase enzyme is obtained from
Thiobacillus ferroxidans
Bacillus subtilis
Pseudomonas subtilis
Pseudomonas subtilis
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
centrifugation
polymerase chain reaction
electrophoresis
electrophoresis
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
Bacteria -Lysozyme
Plant cells - Cellulase
Algae - Methylase
Algae - Methylase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Non-recombinant bacteria containing β-galactosidease
Insertional inactivation of α - galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
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