Short Answer TypeHow has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in the correct sequence.
Explain the roles of the following with the help of an example each in recombinant DNA technology:
(a) Restriction Enzymes
(b) Plasmids
Multiple Choice QuestionsWhich of the following is not a feature of the plasmids?
Circular structure
Transferable
Single stranded
Single stranded
The Taq polymerase enzyme is obtained from
Thiobacillus ferroxidans
Bacillus subtilis
Pseudomonas subtilis
Pseudomonas subtilis
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
centrifugation
polymerase chain reaction
electrophoresis
electrophoresis
B.
polymerase chain reaction
C.
electrophoresis
DNA fragments generated by the reaction is simply DNA replication in a test tube. Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes, e.g., endonucleases that recognise specific 4 to 8 base regions of DNA.
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
Bacteria -Lysozyme
Plant cells - Cellulase
Algae - Methylase
Algae - Methylase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Non-recombinant bacteria containing β-galactosidease
Insertional inactivation of α - galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
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