Short Answer TypeHow has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in the correct sequence.
How has the development of bioreactor helped in biotechnology?
Small volume cultures cannot yield appreciable quantities of products. To produce in large quantities, the development of bioreactors, where large volumes (100-100 litres) of culture can be processed, was required. Thus, bioreactors can be thought of as vessels in which raw material are biologically converted into specific products, individual enzymes, etc., using the microbial plant, animal or human cells.
Explain the roles of the following with the help of an example each in recombinant DNA technology:
(a) Restriction Enzymes
(b) Plasmids
Multiple Choice QuestionsWhich of the following is not a feature of the plasmids?
Circular structure
Transferable
Single stranded
Single stranded
The Taq polymerase enzyme is obtained from
Thiobacillus ferroxidans
Bacillus subtilis
Pseudomonas subtilis
Pseudomonas subtilis
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
centrifugation
polymerase chain reaction
electrophoresis
electrophoresis
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
Bacteria -Lysozyme
Plant cells - Cellulase
Algae - Methylase
Algae - Methylase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Non-recombinant bacteria containing β-galactosidease
Insertional inactivation of α - galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
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