Short Answer TypeHow has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in the correct sequence.
Name the most commonly used bioreactor and describe its working.
The most commonly used bioreactors are of stirring type. A stirred - tank reactors is usually cylindrical or with a curved base to facilitate the mixing of the reactor contents. The stirrer facilitates even mixing and oxygen availability throughout the bioreactor. The bioreactor has an agitator system, an oxygen delivery system and a foam control system, a temperature control system. pH control system and sampling ports so that small volumes of the culture can be withdrawn periodically.
Explain the roles of the following with the help of an example each in recombinant DNA technology:
(a) Restriction Enzymes
(b) Plasmids
Multiple Choice QuestionsWhich of the following is not a feature of the plasmids?
Circular structure
Transferable
Single stranded
Single stranded
The Taq polymerase enzyme is obtained from
Thiobacillus ferroxidans
Bacillus subtilis
Pseudomonas subtilis
Pseudomonas subtilis
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
centrifugation
polymerase chain reaction
electrophoresis
electrophoresis
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
Bacteria -Lysozyme
Plant cells - Cellulase
Algae - Methylase
Algae - Methylase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Non-recombinant bacteria containing β-galactosidease
Insertional inactivation of α - galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
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