Explain enzyme-replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
(a) Why must a cell be made 'competent' in biotechnology experiments ? How does
calcium ion help in doing so ?
(b) State the role of 'biolistic gun' in biotechnology experiments.
(A) It is necessary to make cell competent in order to enhance the efficiency of cell to take up foreign DNA easily.
When the cell is treated with a specific solution of divalent cation calcium. It increases the efficiency of the cell to take up the foreign DNA through the pores in the cell wall.
(B) Biolistics and Gene gun is one of the methods to transfer the foreign DNA into the host.
In this method the suitable plant cells are bombarded with high-velocity micro-particles
of gold & tungsten coated with DNA.
How has RNAi technique helped to prevent the infestation of roots in tobacco plants by a nematode Meloidegyne incognita?
Biotechnologists refer to Agrobacterium tumifaciens as a natural genetic engineer of plants. Give reasons to support the statement.
Scientists have succeeded in recovering healthy sugarcane plants from a diseased one.
1. Name the part of the plant used as explant by the scientists.
2. Describe the procedure the scientists followed to recover the healthy plants.
3. Name this technology used for crop improvement.
How does the application of the fungal genus, Glomus, to the agricultural farm increase the farm output?