Short Answer TypeExplain enzyme-replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
(a) Why must a cell be made 'competent' in biotechnology experiments ? How does
calcium ion help in doing so ?
(b) State the role of 'biolistic gun' in biotechnology experiments.
How has RNAi technique helped to prevent the infestation of roots in tobacco plants by a nematode Meloidegyne incognita?
The introduction of DNA using the Agrobacterium vectors into the host plant produced both sense and anti-sense RNA in the host cells. The RNAi (RNA interference method was initiated as the two RNA's were complementary to each other and formed double-stranded RNA. The dsRNA silenced the specific mRNAof the nematode and thus the parasite could not survive in the transgenic host plant. The plant was therefore protected from the parasite.
Biotechnologists refer to Agrobacterium tumifaciens as a natural genetic engineer of plants. Give reasons to support the statement.
Scientists have succeeded in recovering healthy sugarcane plants from a diseased one.
1. Name the part of the plant used as explant by the scientists.
2. Describe the procedure the scientists followed to recover the healthy plants.
3. Name this technology used for crop improvement.
How does the application of the fungal genus, Glomus, to the agricultural farm increase the farm output?
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