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Molecular Basis of Inheritance

Short Answer Type

31. (a) How did the transformation experiments of Griffith differ from those of Avery and MacCleod ?
(b) What was the significance of each ?

(a) Griffith’s in vivo experiments demonstrated the occurrence of transformation in Pneumococcus. They provide no indication as to molecular basis of the transformation phenomenon. Avery and MacCleod carried their experiments in vitro employing biochemical analysis to demonstrate that transformation was mediated by DNA

(b) Griffith showed that a transforming substance existed whereas Avery, MacLeod and McCarty proved that it is DNA.

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32. What was the rationale of using ³²P and ³⁵S by Hershey and Chase ? Instead, if we use radiolabelled C and N, will the results be any different ?

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33. Which three components that make up the nucleotides ?

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34.

Draw a diagram of polynucleotide chain.

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35. Why is the DNA molecule compared to a spiralling staircase ?

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Long Answer Type

36.

Sketch a double helix of DNA.

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Short Answer Type

37.

If a double stranded DNA has 20 per cent of cytosine, calculate the percent of adenine in the DNA.

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38. If the sequence of one strand of DNA is written as follows : 5’–ATGCATGCATGCATGCATGCATGCATG C –3’ Write down the sequence of complementary strand in 5’ and 3’ direction.

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