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Molecular Basis of Inheritance

Short Answer Type

31. (a) How did the transformation experiments of Griffith differ from those of Avery and MacCleod ?

(b) What was the significance of each ?

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32. What was the rationale of using ³²P and ³⁵S by Hershey and Chase ? Instead, if we use radiolabelled C and N, will the results be any different ?

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33. Which three components that make up the nucleotides ?

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34.

Draw a diagram of polynucleotide chain.

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35. Why is the DNA molecule compared to a spiralling staircase ?

DNA molecule is “Double helical” structure. It is formed of two unbranched polynucleotide complementary strands. The two strands are spirally coiled around a central axis. The nitrogen bases are paired similar to horizontal steps of ladder whereas sugar and phosphate looks similar to vertical bars. Thus the DNA is compared to a spiralling staircase.

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Long Answer Type

36.

Sketch a double helix of DNA.

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Short Answer Type

37.

If a double stranded DNA has 20 per cent of cytosine, calculate the percent of adenine in the DNA.

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38. If the sequence of one strand of DNA is written as follows : 5’–ATGCATGCATGCATGCATGCATGCATG C –3’ Write down the sequence of complementary strand in 5’ and 3’ direction.

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39. If the sequence of coding strand in a transcription unit is written as follows :

5’– ATGCATGCATGCATGCATGCATGCATGC –3’ Write down the sequence of mRNA.

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40. What are Chargaff rules ?

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