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Molecular Basis of Inheritance

Long Answer Type

81. Mention the steps of translation.

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82. Write salient features of human genome project.

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83. List the steps involved in DNA fingerprinting.

Steps of DNA Fingerprinting are :

(i) DNA is isolated.
(ii) DNA is digested by restriction endonucleases,
(iii) The fragments od DNA are seperated by electrophoresis,
(iv) The fragments are then traferred to to synthetic membranes, such as nitrocellulose or nylon.
(v)It is hybridized using labelled VNTR probe.
(vi) Hybridised DNA fragments are detected by autoradiography.

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Short Answer Type

84.

Briefly describe the following:
(a) Transcription
(b) Polymorphism
(c) Translation
(d) Bioinformatics

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Long Answer Type

85.

Describe Griffith’s experiment to demonstrate that DNA is the basic genetic material. What observation was given by Avery, McCarty and MacCleod?

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86.

How is long DNA molecule adjusted in a nucleus.

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Short Answer Type

87. Write a note on semiconservative mode of DNA replication.

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88. Name two major enzymes of DNA replication ?

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89. One of the codons on mRNA is AUG.What is unique about this code

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Long Answer Type

90.

What is the inducer in the lac operon? How does it ensure the 'switching on' of genes?
Draw a schematic representation of Lac operon of how does this operon get switched ‘on’ or ‘off.
Or
Explain how the gene expression is regulated?

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