Briefly describe the following:
(a) Transcription
(b) Polymorphism
(c) Translation
(d) Bioinformatics
Describe Griffith’s experiment to demonstrate that DNA is the basic genetic material. What observation was given by Avery, McCarty and MacCleod?
What is the inducer in the lac operon? How does it ensure the 'switching on' of genes?
Draw a schematic representation of Lac operon of how does this operon get switched ‘on’ or ‘off.
Or
Explain how the gene expression is regulated?
Inducer. It is a chemical may be a substrate, hormone or some other metabolite which after coming in contact with the repressor, changes the latter into non-DNA binding state so as to free the operator gene. Thus the “switch on” occurs.
The expression of the genes is usually controlled to achieve maximum cellular economy. This means that gene will be turned on or off as per requirement. A set of genes will be switched on when there is necessity to handle and metabolise a new substrate. When these genes are turned on enzymes are produced, which metabolize the new substrate. The phenomenon is known as induction.
Lac operon