Short Answer TypeTaq polymerase is obtained from the bacterium Thermus aquaticus. It is a thermostable DNA polymerase enzyme and remains active even remain active during the high temperature induced denaturation of double stranded DNA in a PCR reaction.
(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play.
(b) Why is the coding sequence of an enzyme 
-galactosidase a preferred selectable marker in comparison to the ones named above ?
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Long Answer TypeA. Describe the characteristics a cloning vector must possess.
B. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium?
If a desired gene is identified in an organism for some experiments, explain the process of the following:
(i) Cutting this desired gene at specific location
(ii) Synthesis of multiple copies of this desired gene
Short Answer Type
Switch
