Short Answer Type(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play.
(b) Why is the coding sequence of an enzyme 
-galactosidase a preferred selectable marker in comparison to the ones named above ?
Sugggest a technique to a researcher who needs to separate fragments of DNA.
The researcher can use the method Electrophoresis (Gel electrophoresis to separate the fragments of the DNA. In this method, the DNA fragments are loaded into the well made in the gel and separated according to their size under the influence of an electric current.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Long Answer TypeA. Describe the characteristics a cloning vector must possess.
B. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium?
If a desired gene is identified in an organism for some experiments, explain the process of the following:
(i) Cutting this desired gene at specific location
(ii) Synthesis of multiple copies of this desired gene
Short Answer Type
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