Short Answer Type(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play.
(b) Why is the coding sequence of an enzyme 
-galactosidase a preferred selectable marker in comparison to the ones named above ?
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
The technique to obtain multiple copies of a gene is PCR - Polymerase Chain rection.
It invoves the following steps.
Denaturing – The double-stranded template DNA is heated to separate it into two single strands.
Annealing – The temperature is lowered to enable the DNA primers to attach to the template DNA.
Extending – The temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.
These three stages are repeated 20-40 times, doubling the number of DNA copies each time.
Long Answer TypeA. Describe the characteristics a cloning vector must possess.
B. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium?
If a desired gene is identified in an organism for some experiments, explain the process of the following:
(i) Cutting this desired gene at specific location
(ii) Synthesis of multiple copies of this desired gene
Short Answer Type
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