What does EcoRI signify ? How its name is derived ? from Biology

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21. What does EcoRI signify ? How its name is derived ?


EcoRI signifies the name of restriction endonuclease derived from bacterium Escherichia coli.

The name is obtained using the conventaional method -:

(i) First capital letter of the name comes from the genus of the organism Escherichia is ‘E’.

(ii) Second two small letters comes from the species of cells from which they are isolated i.e. ‘co’.

(iii) Letter R is derived from the name of strain  of the bacteria i.e. Escherichia coli Ry_ 13.

(iv) Roman number indicate the order in which enzymes were isolated from that strain of bacteria .

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22. Give the source of restriction enzyme, Bam HI and Kpn I.
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23. What are recognition sequences or recognition sites ?
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24. How are restriction endonuclease enzymes named ?
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25. Explain any three methods of vectorless gene transfer.
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26. Define vector. Give the properties of a “Good Vector”.
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27. Write a note on cloning vector.
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28. What do you understand by the term selectable marker ?
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 Multiple Choice QuestionsLong Answer Type

29. Give the overall steps involved in Gene cloning.
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