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21. What does EcoRI signify ? How its name is derived ?

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22. Give the source of restriction enzyme, Bam HI and Kpn I.

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23. What are recognition sequences or recognition sites ?

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24. How are restriction endonuclease enzymes named ?

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25. Explain any three methods of vectorless gene transfer.

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26. Define vector. Give the properties of a “Good Vector”.

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27. Write a note on cloning vector.

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28. What do you understand by the term selectable marker ?

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29. Give the overall steps involved in Gene cloning.

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(i) PCR. Polymerase Chain Reaction.

(ii) Three steps of PCR

1. Denaturation

2. Primer annealing and

3. Extension of primers.

The three steps of PCR

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