Give the source of restriction enzyme, Bam HI and Kpn I. from Bio
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    Biotechnology : Principles and Processes

    Multiple Choice QuestionsShort Answer Type

    21. What does EcoRI signify ? How its name is derived ?
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    22. Give the source of restriction enzyme, Bam HI and Kpn I.

    Kpn I is obtained from Klebsiella pneumoniae and Bam HI is obtained from Bacillus amyloliquefaciens .
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    23. What are recognition sequences or recognition sites ?
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    24. How are restriction endonuclease enzymes named ?
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    25. Explain any three methods of vectorless gene transfer.
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    26. Define vector. Give the properties of a “Good Vector”.
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    27. Write a note on cloning vector.
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    28. What do you understand by the term selectable marker ?
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    Multiple Choice QuestionsLong Answer Type

    29. Give the overall steps involved in Gene cloning.
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    30. What is PCR? List the three main steps. Show the steps with a diagrammatic sketch.
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