Describe briefly the following : (a) Origin of replication (b) Bi

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 Multiple Choice QuestionsLong Answer Type

41.

What are bioreactors?. What is the utility?  Draw the digram of the two types of bioreactors

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42. Describe briefly the following :

(a) Origin of replication (b) Bioreactor (c) Downstream processing


(a) Origin of replication (Ori).
1. It is a specific sequence of DNA bases, which  initiates replication.A prokaryotic DNA has normally a single oringin of replication, while eukaryotic DNA may have more than one. This sequence controls the copy number of linked DNA. An alien DNA for replication should be linked to the origin of replication

(b) Bioreactor are vessels in which raw materials are biologically converted into specific products using microbial, plant or human cells. It provides optimal conditions for achieving the desired product by providing optimum growth conditions, pH, substrate salts, vitamins, oxygen, etc.

(c) Downstream processing.are the series of processes which a genetically modified product undergoes before it is ready to be marketed. It generally involves processes like  separation and  purification.

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 Multiple Choice QuestionsShort Answer Type

43. Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks ?
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 Multiple Choice QuestionsLong Answer Type

44. Explain briefly the following

(a) PCR (b) Restriction enzymes and DNA

(c) Chitinase.

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 Multiple Choice QuestionsShort Answer Type

45. Discuss with your teacher and find out how to distinguish between

(a) Plasmid DNA and chromosomal DNA
(b)Exonuclease and endonuclease.

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46. Can you list 10 recombinant proteins which are used in medical practice ? Find where they are used as therapeutics (use the internet).
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47.

Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.

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48. Name the technique used for separation of DNA fragments. What is its principle ? How are they observed ?
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49.

What are molecular scissors. Explain their role.
Or
How does restriction endonuclease work ?

 

 

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 Multiple Choice QuestionsLong Answer Type

50. How is DNA isolated in purified form ?
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