What are bioreactors?. What is the utility? Draw the digram of the two types of bioreactors
(a) Origin of replication (b) Bioreactor (c) Downstream processing
(a) Plasmid DNA and chromosomal DNA
(b)Exonuclease and endonuclease.
Plasmid DNA |
Chromosomal DNA |
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1. It is self replicating, DNA molecule found naturally in many bacteria and yeast. 2. It is not essential for normal growth and division and contains information for a few traits. |
1. Chromosomal DNA present in chromosomes of all organisms. 2. It is essential for growth and division and contains information for all traits. |
(b) Difference between exonuclease and endonuclease
Exonuclease This enzyme removes nucleotides from the terminals ,either from 5' or 3' ends of DNA molecules. |
Endonuclease It cuts the DNA at specific position of nitrogen bases within the length of DNA except the ends. |
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
What are molecular scissors. Explain their role.
Or
How does restriction endonuclease work ?