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41.

What are bioreactors?. What is the utility? Draw the digram of the two types of bioreactors

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42. Describe briefly the following :

(a) Origin of replication (b) Bioreactor (c) Downstream processing

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43. Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks ?

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44. Explain briefly the following

(a) PCR (b) Restriction enzymes and DNA

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45. Discuss with your teacher and find out how to distinguish between

(a) Plasmid DNA and chromosomal DNA
(b)Exonuclease and endonuclease.

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46. Can you list 10 recombinant proteins which are used in medical practice ? Find where they are used as therapeutics (use the internet).

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48. Name the technique used for separation of DNA fragments. What is its principle ? How are they observed ?

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49.

What are molecular scissors. Explain their role.
Or
How does restriction endonuclease work ?

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50. How is DNA isolated in purified form ?

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