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Biotechnology : Principles and Processes

Long Answer Type

41.

What are bioreactors?. What is the utility? Draw the digram of the two types of bioreactors

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42. Describe briefly the following :

(a) Origin of replication (b) Bioreactor (c) Downstream processing

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Short Answer Type

43. Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks ?

305 Views

Long Answer Type

44. Explain briefly the following

(a) PCR (b) Restriction enzymes and DNA

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Short Answer Type

45. Discuss with your teacher and find out how to distinguish between

(a) Plasmid DNA and chromosomal DNA
(b)Exonuclease and endonuclease.

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46. Can you list 10 recombinant proteins which are used in medical practice ? Find where they are used as therapeutics (use the internet).

Recombinant Protein

Therapeutic Use

1. Insulin

2. Human Growth Hormone

3. Interferons alpha

4. Recombinant Clotting factor VIII

5. Anti thrombin III

6. Erythropoietin

7. DNase I

8. Interleukin 2

9. Granulocyte macrophage Colony stimulating factor

10. Bovine growth hormone

1. For the treatment of insulin dependent diabetes

2.For the treatment of dwarfism

3.For treating hepatitis C

4.For treating haemophilia

5.For prevention of blood clots

6.For treatment of Anaemia

7.For treating Cystic Fibrosis

8.For treatment of cancers

9.For treating cancer

10.For increasing milk yield.

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47.

Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.

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48. Name the technique used for separation of DNA fragments. What is its principle ? How are they observed ?

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49.

What are molecular scissors. Explain their role.
Or
How does restriction endonuclease work ?

1337 Views

Long Answer Type

50. How is DNA isolated in purified form ?

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