Long Answer TypeWhat are bioreactors?. What is the utility? Draw the digram of the two types of bioreactors
(a) Origin of replication (b) Bioreactor (c) Downstream processing
Short Answer TypeLong Answer TypeShort Answer Type(a) Plasmid DNA and chromosomal DNA
(b)Exonuclease and endonuclease.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
What are molecular scissors. Explain their role.
Or
How does restriction endonuclease work ?
Long Answer TypeThe steps to isolate DNA in purified form are -:
1.The cell is broken up to release the DNA molecules along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This is done by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).
2. The RNA is removed by treatment with ribonuclease.
3. The proteins are removed by treatment with protease.
4.Ethanol is added to precipitate the DNA.
5. The purified DNA can be collected in the form of fine threads in the suspension.
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