2. It is used in genetic fingerprinting and forensics.
3. PCR can be used for DNA sequencing .
4. It is used for detecting diseases.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.