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31. Give the applications of PCR technology.

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32. Why is “Agrobacterium mediated genetic transformation” in plants described as Natural genetic engineering of plants ?

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33. Name the various cloning vectors and explain how a plasmid can be used for genetic engineering.

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34. How is recombinant DNA transferred to host ?

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Action of restriction enzyme

EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA.

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36. List the steps in the formation of rDNA.

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37. How is isolated gene of interest amplified ?

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38.

List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.

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39. Explain the importance of (a) ori, (b) amp^R and (c) rop in the E. coli vector .

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40. Name any two cloning vectors.

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