Why is “Agrobacterium mediated genetic transformation&rdquo

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 Multiple Choice QuestionsShort Answer Type

31. Give the applications of PCR technology.
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32. Why is “Agrobacterium mediated genetic transformation” in plants described as Natural genetic engineering of plants ?


Agrobacterium tumefaciens cause the crown gall disease  in most of the dicotyledonous plants. These bacteria contain large tumors inducing plasmid (Ti–plasmids) . A part called T-DNA of this Ti plasmid is transferred to the host which transforms the normal plant cells into tumor cells and directs them to produce chemicals needed by the pathogen . The gene transfer  and transformation is brought about naturally without any artificial methods thus 'Agrobacterium mediated genetic transformation' is said to be Natural genetic engineering of  plants.
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33. Name the various cloning vectors and explain how a plasmid can be used for genetic engineering.
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 Multiple Choice QuestionsLong Answer Type

34. How is recombinant DNA transferred to host ?
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 Multiple Choice QuestionsShort Answer Type

35.

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

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36. List the steps in the formation of rDNA.
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37. How is isolated gene of interest amplified ?
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 Multiple Choice QuestionsLong Answer Type

38.

List the features required in vectors that  facilitate cloning . With a suitable diagram show the E.coli cloning vector with  restriction sites.

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 Multiple Choice QuestionsShort Answer Type

39. Explain the importance of (a) ori, (b) ampR and (c) rop in the E. coli vector .
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40. Name any two cloning vectors.
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