Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Recombined DNA technology involves the following steps :
(i) Isolation of DNA.
(ii) Cutting of DNA by restriction endonucleases.
(iii) Isolation and amplification of the gene of interest.
(iv) Ligation of the DNA fragment into a vector using DNA ligase.
(v) Transfer of DNA fragment into host cell.
(v) Culturing of host cell and extraction of the recombinant product.
List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.