1. Plasmids.
2. Bacteriophages.
3. Plant vectors like Agrobacterium tumefaciens and animal vectors likeand retroviruses.
4.Artificial chromosomes of bacteria and yeast like BACs and YACs.
Plasmid can be used as a cloning vector in genetic engineering. Since they have the capacity to replicate autonomously (independently) and may have a high copy number , foreign DNA can be linked to it and multiple copies of the desired gene can be obtained.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.