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Biotechnology : Principles and Processes

Short Answer Type

31. Give the applications of PCR technology.

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32. Why is “Agrobacterium mediated genetic transformation” in plants described as Natural genetic engineering of plants ?

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33. Name the various cloning vectors and explain how a plasmid can be used for genetic engineering.

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Long Answer Type

34. How is recombinant DNA transferred to host ?

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Short Answer Type

35.

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

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36. List the steps in the formation of rDNA.

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37. How is isolated gene of interest amplified ?

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Long Answer Type

38.
List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.

Features required to facilitate cloning vector.

1. Origin of replication (Ori)

2. Selectable marker

3. Cloning sites

4. Vectors for cloning genes in plants and animals Sites of cloning vector

coli Cloning Vector pBR322 showing restriction sites (Hindlll, EcoRI, BamHI, Sal I, Pvu II, Pst I, Clal), oriV and antibiotic resistance genes (ampR and tetR). Rop codes for the proteins involved in the replication of the plasmid.

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