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Biotechnology : Principles and Processes

Short Answer Type

31. Give the applications of PCR technology.

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32. Why is “Agrobacterium mediated genetic transformation” in plants described as Natural genetic engineering of plants ?

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33. Name the various cloning vectors and explain how a plasmid can be used for genetic engineering.

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Long Answer Type

34. How is recombinant DNA transferred to host ?

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Short Answer Type

35.

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

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36. List the steps in the formation of rDNA.

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37. How is isolated gene of interest amplified ?

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Long Answer Type

38.

List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.

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Short Answer Type

39. Explain the importance of (a) ori, (b) amp^R and (c) rop in the E. coli vector .

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40. Name any two cloning vectors.

Cloning vectors are used for propagation of DNA inserts in a suitable host . Examples of cloning vectors are
pBR322 an E.coli cloning vectors and Agrobacterium tumefaciens a plant cloning vector.

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