Short Answer Type
Long Answer TypeShort Answer TypeMake a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
(i) Denaturation in which the double-stranded DNA is denatured by using high temperatures
(ii) Primer annealing - in this step two sets of primers are used that are complementary to the segment of DNA of interest. the lower temperature favors the attachment or annealing of primer to the DNA strands.
(iii) Extension of primers in which DNA polymerase enzyme is used to make copies of DNA .
Long Answer TypeList the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.
Short Answer Type
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