Short Answer Type
Long Answer Type1. The bacterial cells are made competent to take up recombinant DNA by treating them with a specific concentration of calcium, that increases the efficiency with which DNA enters the cell through the pores in its cell wall.
2. Microinjection is a method in which the recombinant DNA is directly injected into the nucleus-of the animal cell with the help of mirco-needles or micropipettes.
3. Gene gun or biolistics is a method suitable for plant cells, where plant cells are bombarded with high-velocity microparticles of gold or tungsten coated with DNA.
4. Disarmed pathogens can be allowed to infect the host cell, which then transfer the recombinant DNA into the host.
5. Electroporation in which electricity is applied to create transient pores in the host cell so that it takes up Recombinant DNA with ease.
Short Answer TypeMake a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Long Answer TypeList the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.
Short Answer Type
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